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To determine the cellular expression pattern of the choline transporters throughout gestation, immunofluorescence analysis was then performed.Both CTL1 and CTL2 were expressed in the chorionic villi from 6 weeks gestation to term.
This widespread cellular expression pattern suggests SLO-1 performs an important role in regulating the activity of the pharyngeal network.
Having established the cellular expression pattern of Sez-6 in the rat retina, we carried out morphological, immunohistochemical and electrophysiological analyses of Sez-6 knockout mouse retina.
These results were consistent with previously published data showing that the cellular expression pattern of Cx26 is unaltered in the cochlear sections of Cx30 null mice [7], [8].
Future analyses of BHMT cellular expression pattern may provide more clues to its function.
(B ) The cellular gene set was subcategorized according to the specific cellular expression pattern.
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In contrast, temporal and cellular expression patterns of PLD4 mRNA are fairly restricted to developing mouse brains and various tissues.
We compared our microarray data sets with previously reported cellular expression patterns of each retinal gene obtained from published in situ hybridization data [37].
The aims of this study were to characterize the cellular expression patterns of Sez-6 using a specific Sez-6 polyclonal antibody able to detect the multiple isoforms and to investigate the function of Sez-6 in the rodent retina.
This notion is directly supported by data showing that the cellular expression patterns of Cx30 and Cx26 in the cochlea of Cx26 and Cx30 null mice, respectively, are unaltered [7], [8].
Furthermore, we compared our microarray data with previously reported cellular expression patterns of each retinal gene obtained from published results of in situ hybridization [37], [38], immunohistochemistry [39], [40], and cell type specific GFP expression in the retina using BAC transgenic mice from the GENSAT project [41] (Table 1 and 2).
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