Three ISTH SSC (Vascular Biology, Disseminated Intravascular Coagulation, and Haemostasis and Malignancy) have initiated a project aiming at standardization of the enumeration of cellular MP by flow cytometer.
Measurement data (levels of mRNA and protein, the data of cellular migration and proliferation) and enumeration data (the data of immunohistochemical staining) were respectively analyzed using the paired t-test and the Fisher's exact test (two-tailed).
Besides microscopic enumeration and morphological examination, cellular biomarkers appear promising.
Similarly, enumeration of all the cancer-related cellular components would identify all the genes that are likely related to the expression of cancerous cellular phenotype.
Bactericidal effect of CPV on MRSA and VISA infected keratinocyte cells was examined by enumeration of extra- and intra-cellular bacterial cells at different treatment time points.
The enumeration of the interaction partners of the cellular prion protein, PrPC, may help clarifying its elusive molecular function.
This assay has been shown to be suitable for enumeration of low frequencies of antigen specific cellular responses following in vivo priming with antigen [ 56].
The structure appeared within 12 h, and further incubation resulted in its growth into a large cellular aggregate due to reiterative internalization, which hampered the accurate enumeration of internalization ratio after 24 h.
For most cellular populations 20,000 events were analyzed; however, 50,000 events were examined for enumeration of activated B cells and 200,000 lymphoid events for quantitation of NKT and Treg cells.
This platform can perform label-free and rapid single-cell capture, efficient cellular manipulation, rare-cell isolation, selective analytical separation of biological species, sorting, concentration, positioning, enumeration, and characterization.
Enumeration was completed upon identification of 25 abnormal cells or the complete analysis of the cellular area.
