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As previously proposed37,38,39, cellular contractility is also dependent of the spatial organization of stress fibres, with EVL being a key player in this context.
This study reveals the important synergistic roles of cellular contractility and tissue stiffness in the maintenance of fibrotic tissue and suggests a new therapeutic principle for fibrosis.
Cellular contractility was quantified using a valve thin film assay and phenotype analyzed via western blotting, zymography, and qRT-PCR.
To develop a quantitative perspective on how cellular contractility, scaffold curvature and cell-substrate adhesion control such rupture processes, human aortic smooth muscle cells were grown on glass substrates with submillimeter semichannels.
Cellular contractility was highest for the most elongated cells, but was also increased in cells on the widest pattern (80 μm) that also had increased CX43 expression, suggesting a role for both elongated shape and increased cell-cell contact in regulating contractility.
Cellular mechanics are influenced in part by the combination of cell morphology and cytoskeletal organization with the formation of stress fibers enabling increased cellular contractility [120].
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To assess the scaffold's ability to support cardiac cellular functionality, contractility, and electrical coupling hiPS-CMs were utilized.
In fact, both cellular motility and contractility have previously been well connected to actin expression, and there is also evidence for a particular role for the α-SMA isoform.
Based on cellular viability and contractility, 3 minutes of ischemia was chosen for further experimentation.
Moreover, cGMP regulates several processes, including cellular growth and contractility, cardiovascular homoeostasis, inflammation, sensory transduction, neuronal plasticity and learning [ 59].
Since its discovery, S-nitrosylation has been implicated in the regulation of proteins involved in cellular trafficking, muscle contractility, apoptosis, neural transmission, and host defense.
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