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In order to analyze spliceosome structure, a stringent approach was developed to obtain splicing complexes free of cellular contaminants.
Because pancreatic juice collected clinically sometimes contains the ZG content proteins as well as blood and cellular contaminants (Doyle et al. 2012), proteins found in both datasets represent potential bona fide ZG content proteins.
Sperm were separated from seminal plasma using Isolate® Sperm Separation Medium (Irvine Scientific, Santa Ana, CA), a density gradient centrifugation column designed to separate cellular contaminants (including leukocytes, round cells, and miscellaneous debris) from spermatozoa [30].
Cellular contaminants were removed by centrifugation at 900 g for 10 min prior to storing the supernatant at −80°C for later analysis.
By the injection of a wash buffer, all other cellular contaminants and proteins pass through the channels while the DNA remains bonded to the silica surface.
As a second step, a wash buffer of 100 μL (Invitrogen) was injected to remove all other cellular contaminants and proteins through the pores while DNA remains bound to the silica surface.
Similar(47)
In the case of measles virus, actin was originally thought as a cellular contaminant, but was later demonstrated to be an internal component of the virus [23] [25].
Sera frequently reacted with a 42 kDa protein which is a cellular contaminant of the virus.
The second urine in the morning were collected into a sterile tube and centrifuged at 800 x g for 10 min at 4 °C, in order to remove cellular debris and contaminants.
While mass spectrometry is exquisitely sensitive, subcellular fractionations only provide partial enrichment of cellular components from contaminants.
We speculate that on a per volume basis, the greater availability of defects and contaminants for cellular interaction may contribute to the higher cytotoxicity of SWCNT in this study.
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