The ability to control cell placement and to produce idealized cellular constructs is essential for understanding and controlling intercellular processes and ultimately for producing engineered tissue replacements.
Consequently, controlling the shape of three-dimensional (3D) cellular constructs is an important issue for in vitro applications of fabricated artificial livers.
The formation of cellular constructs was clearly observed via a microscope after 2 day-cultivation.
First, multi-layered cellular construct was fabricated by stacking fibroblast cellular films.
However, most 3D-cellular constructs are a cell spheroid, which is a heterogeneous aggregation, and thus the reconstruction of the delicate and precise 3D-location of multiple types of cells is almost impossible.
Conclusions: Unlike acellular grafts, engineered cellular diaphragmatic constructs are anatomically and histologically similar to normal muscle.
For cellular studies, FLAG-tagged TUBB5 DNA constructs were transfected into Neuro-2a cells before immunostaining with an anti-α-tubulin antibody (Sigma, T6199-200UL) and anti-FLAGLantibodyody (Abcam, ab1162).
To study the effect of these mutations on the cellular localization of Nav1.7, FLAG-tagged SCN9A constructs were generated.
