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After verification of the binding in a yeast two-hybrid assay, the most strongly interacting polypeptide was introduced to different cellular compartments of Arabidopsis cell via genetic transformation.
The dynamic transitions in basement membrane composition during development suggest cochlear basement membranes may function to direct differentiation and/or terminal cytodifferentiation of the complex cellular compartments of the cochlear epithelium.
At higher magnification (4D), the area of intense labeling showed that all of the cellular compartments of the photoreceptors adjacent to the lesions were labeled.
THP-1 cells were infected with mycobacteria and after 48 h of infection mycobacterial colocalization with cellular compartments of infected macrophages was analyzed by immunofluorescence.
The amelioration of the light-induced retinal degeneration in Q344terrho+/−, Trα−/− mice suggests that Q344ter-catalyzed transducin signaling in the inappropriate cellular compartments of outer nuclear layer and inner segment may play a role.
Among the ESTs we identified 12 proteins that are unique to mitochondrial- or plastid-derived organelles and have never been observed in other cellular compartments of eukaryotes (Table 1).
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Fluorescence microscopy observed that these drug carriers could internalize efficiently into the cellular compartment of MCF-7 cells.
A tumor-specific mutation signature was detected in the initial plasma samples from all patients, but in the cellular compartment of blood in only two patients, one of whom subsequently developed metastatic disease (Patient 2).
This explains the different localization patterns of meprinβ and E-cadherin during progression to fully established cell polarity and has direct implications for the cellular compartment of meprinβ-mediated E-cadherin processing.
Caveats to the correlation of the tumor-specific signal in plasma or the cellular compartment of blood with the actual tumor burden in the patient are (i) the possible underestimation of the tumor burden if the somatic tumor mutation is present only in some regions of the tumor and (ii) overestimation of the tumor burden if occasional somatic mosaicism occurs in normal tissue.
The cellular compartment of blood was analyzed as an indicator of circulating tumor cells and the plasma was analyzed as an indicator of apoptotic/necrotic cancer-shedding membrane-encapsulated short DNA fragments in the circulation [23], [24].The mutation frequency was estimated at about 4 molecules per milliliter of plasma at pretreatment and within 24 hours after first treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com