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Cellular binding to fibrinogen and fibrinogen fragment D was measured with an ELISA ([15], Figueira et al).. Briefly, microtiter wells coated with 1 µg purified human fibrinogen or fibrinogen fragment D (both from HYPHEN BioMed) and blocked with 20 mg/mL BSA were incubated with 3×108 leptospires at 30°C for 1 h.
The endometrium is unlikely to play a central role in the pathogenesis of Nme, but restriction of Nme PilC2-mediated adhesion to ME-180 cells could indicate a highly specific modulation of meningococcal cellular binding to yet unrecognised cell types of the nasopharynx.
Furthermore the anti-beta 1 integrin monoclonal antibody (mAb13) inhibits the glandular differentiation of SW1222 cells (61%) and their cellular binding to type I collagen (60%).
Since proMMP-2 activation is directly associated with the level of MT1-MMP activity, these results indicated that cellular binding to BSP via integrin αvβ3 does not modify MT1-MMP activity on the cell surface.
Additionally, GRIK1 can indirectly affect cellular binding to paclitaxel through co-regulation of expression by Atrophin 1 (ATN1), which also regulates Stathmin 1 (STMN1) expression and STMN1 reduces cellular binding of paclitaxel [ 61- 63].
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The mechanisms like extracellular (chelation and cell wall binding) and intra-cellular (binding to compounds like proteins) sequestration of heavy metals have been proposed as mechanisms for heavy metals tolerance in fungi (Fawzy et al. 2017).
Astriab-Fisher, A; Sergueev, D; Fisher, M; Shaw, BR; Juliano, RL, "Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides: effects on cellular uptake, binding to target sequences, and biologic actions"., Pharm Res, 2002, 19 6), 744-754 [Gateway.cgi], [doi] [abs].
Interactions between E6 and its many cellular binding partners contribute to viral propagation and the possible progression to cancer.
The specificity of this interaction was demonstrated by the finding that none of these mutants had an enhanced resistance to a second auxin efflux inhibitor, TIBA, that has a cellular binding site distinct to NPA [ 29, 30].
We used the well-characterized interaction between Anx2 and its cellular binding partner p11 [69] to develop the BiFC assay.
It is further expected that curcuminoids solubilized in sera from different species or possibly different preparations from the same species, will produce different apparent cellular binding KDs due to their variable serum compositions.
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