Here, we used Arc cellular compartmental analysis of temporal gene transcription by fluorescence in situ hybridization (catFISH) to identify neurons activated during single trial contextual fear conditioning in rats.
Finally, the change from the dense cellular staining in hpg ARC, corresponding to elevated Kiss1 gene expression, to a WT pattern of kisspeptin neuropil, suggests E2 restored kisspeptin distribution to a pattern found at low Kiss1 expression.
To investigate the significance of this finding at the functional level, we determined whether pharmacological stimulation of the Gq-coupled 5-HT2CRs was equally effective in increasing ARC POMC cellular activity in brain slices from young adult and middle-aged POMC-EGFP mice.
Arc is a cellular immediate-early gene (IEG) that functions at excitatory synapses and is required for learning and memory.
Although the direct demonstration of an association between Arc signals and cellular activity is still lacking, accumulating evidence suggests that neuronal activity of cells precedes the Arc expression (Lyford et al. 1995; Steward and Worley 2001; Shepherd et al. 2006).
In these regions particularly it is unclear to what extent neuronal activity per se was driving recombination, or whether TAM induced Arc expression via cellular stress as it does expression of Atf3.
Of these GO terms in Table 6, many transcripts encoded proteins involved in cellular metabolic process such as NB-ARC domain containing protein, F-box domain containing protein, zinc ion binding proteins and calcium-dependent protein kinase isoform AK1.
In control monolayers without any treatment, ZO-1 was restricted to cellular borders and distributed in a smooth arc-like pattern.
Following ACTH injection, c-Fos immunoreactivity was quantified in key appetite-associated hypothalamic nuclei including the ventromedial hypothalamus (VMH), dorsomedial hypothalamus, lateral hypothalamus (LH), arcuate nucleus (ARC) and the parvo- and magno-cellular portions of the paraventricular nucleus.
The LI group did show significant nuclear Arc in response to the shock, but this cellular response to the US was not as strong as in DS animals.
Taken together, evidence suggests that Arc interacts with several distinct proteins to regulate multiple cellular processes.
