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In the case of cellular analysis using monoclonal antibodies in particular, there is an urgent demand for label-free detections of antigen-antibody reactions at the surfaces of living biological cells.
Apoptosis occurred in this cell line, as assessed by staining of chromosomal DNA with propidium iodide (PI) followed by cellular analysis using the FACScalibur flow cytometer (Figures 1A and S2).
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Cellular expression analysis using the monoclonal antibodies from an international workshop on HLA indicates that CD147 is broadly expressed on haemopoietic and non-haemopoietic cell lines [ 11].
Molecular and cellular functional analysis using Ingenuity Pathway Analysis (IPA) found these genes to be associated with lipid and carbohydrate metabolism, small molecule biochemistry, cell cycle and gene expression based on a Fisher's Exact Test p-value < 0.01.
The limitations of the cellular radio network analysis using the traditional drive test and frequency scanning technology were discussed, which led to the inaccurate interference location and inefficient interference optimization.
The aliquot of cells was prepared for FACS analysis using the cellular DNA flow cytometric analysis kit (Roche, IN) following the manufacturer's specifications.
To compare the subcellular distribution between the three translational isoforms of C/EBPα, we examined their cellular localization by fluorescence microscopic analysis using the Zeiss ApoTome system.
GO analysis using the cellular component ontology indicated that mutants with increased Rnr3 abundance are significantly enriched for genes that reside within the nucleus (Table S7).
As we observe a prominent enrichment for protein complex related processes, we also describe in the following the results of the GPEA S. cerevisiae GRN-PPN interface analysis using Gene Ontology cellular component terms.
To evaluate the use of the GlcNAcstatins for cell biological studies, HEK-293 cells were exposed to various concentrations of GlcNAcstatins for 6 h, followed by investigation of O-GlcNAc levels on cellular proteins by Western blot analysis using an anti- O-GlcNAc antibody (CTD110.6).
As shown in Fig. 1A and B, western blot analysis using total cellular extracts and relative quantification revealed an increase in CSB protein levels in cancer cells (MGH, USB, HeLa, PC3, T47D and MCF7), compared to normal cells (C3PV, RWPE1 and MCF 10A).
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