Cellular analyses indicate that D2/SMG11 controls grain size by promoting cell expansion.
The formed EBs were then plated on gelatin-coated plates to allow spontaneous differentiation and cell outgrowth from EBs. Microscopic analyses showed that cellular outgrowth from Cln1/5 dko EBs was much slower than from the wild-type EBs.
Cellular fractionation and mass spectrometric analyses showed LCL-30 to be enriched in the mitochondrial fraction, in line with published data on cationic ω-pyridinium analogues of C6 and C16 ceramide (Novgorodov et al, 2005; Dindo et al, 2006; Senkal et al, 2006).
After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components.
Numerical sensitivity analyses showed that the cellular deformation under substrate stretching, that is, displacement boundaries, is insensitive to the absolute value of the elastic modulus, but depends on the nuclear to cytoplasmic modulus ratio.
DOI: http://dx.doi.org/10.7554/eLife.08712.012 Lipidomic analyses showed no significant change in cellular ergosterol levels in vps53Δ cells.
Gene ontology analyses showed that a number of key cellular processes are differentially regulated between the lines (Additional file 4: Table S3).
Further protein analyses showed that the analogues had anti-cellular proliferation, pro-apoptosis, and cell cycle arrest properties associated with suppressed EGFR expression.
Quantitative biochemical analyses showed that compared to sham, SCI significantly lowered GSH content accompanied by increased NO production in both cellular and mitochondrial fractions.
Further GO analyses showed that genes with enhancers that were hypermethylated in gametes, but hypomethylated in 6-week embryos were highly enriched in cellular component movement, locomotion and migration (Fig. 5b green box and Supplementary Table S4).
