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Controlling cellular alignment and elongation has been demonstrated as an important parameter for developing nerve tissue engineering scaffolds.
The development of tissue engineering field entails the creation of micro/nanoscale features for cellular alignment and biocompatibility improvement.
We show that micropatterned GelMA scaffolds are non-toxic, produce organized cellular alignment, and promote meniscus-like tissue formation.
The cell interaction between the prescribed microscale patterns of nanofibers and macroscale specimen was experimentally observed with particular focus on cellular alignment and associated tissue architecture[20].
Intriguingly, in vitro bone marrow stem cell staining results showed that such a porous scaffold with anisotropic structure exhibited very high ability to guide cellular alignment and elongation compared to the regular one with isotropic structure.
The presented system may be used as an in vitro model for investigating cell and tissue morphogenesis in 3D, as well as for creating tissue constructs with microscale control of 3D cellular alignment and elongation, that could have great potential for the engineering of functional tissues with aligned cells and anisotropic function.
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Our highly anisotropic RJS fibers are therefore able to support cellular alignment, maturation and self-organization.
A two-tailed t test was used to compare cellular alignment between unpatterned and patterned samples followed by a post hoc Mann–Whitney Rank Sum Test when normality criteria were not met.
Specifically, we find roles for the kinases GSK3 and aPKC in cellular alignment, asymmetric protein distribution, and adhesion during the development of this polarized tissue.
Similar trends were observed upon calculation of the fiber and cellular alignment on the scaffolds, where the AL and CO scaffolds supported highly aligned fibers and cells, whereas the NA scaffold did not (Figure 5B C).
PDMS microgrooved culture substrates brought about cellular alignment (p < 0.0001) and more organised sarcomere.
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