Exact(44)
Similar trends were observed upon calculation of the fiber and cellular alignment on the scaffolds, where the AL and CO scaffolds supported highly aligned fibers and cells, whereas the NA scaffold did not (Figure 5B C).
A 2D FFT method was used to quantify cellular alignment.
Groove widths significantly influenced cellular alignment, with 50 μm grooved patterns affecting alignment most substantially.
Controlling cellular alignment is critical in engineering intestines with desired structure and function.
PDMS microgrooved culture substrates brought about cellular alignment (p < 0.0001) and more organised sarcomere.
Our highly anisotropic RJS fibers are therefore able to support cellular alignment, maturation and self-organization.
Similar(16)
Cellular eccentricity and alignment were measured as the eccentricity and alignment of the ellipse that has the same second moments as the pixel positions of the binarized image of the tracked cells.
In all cases, natural drawn spider silk acted as an effective substrate for cellular adhesion and alignment of DPSCs and could be used in neural differentiation applications.
These concentration-dependent alterations include changes in surface characteristics, cellular shape, and alignment.
Meanwhile, the change in the substrate topography alone induces transformations in the shape, cellular orientation, cell alignment, and elongation.
These data are in agreement with previously reported studies of cellular elongation and alignment 34, 44 and form the basis for comparison with cells subjected to heparan sulfate degradation.
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