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His team had coaxed them to evolve into heart cells, which were grown into a thin, beating layer over an integrated circuit.
Amgen then spliced the human Epo gene into hamster ovary cells, which were grown in culture and could churn out large quantities of the protein to use as a drug.
N-terminal His-tagged DnaK (His-DnaK) was overexpressed from pDnaKWT (ASKA clone60) in E. coli BL21 (DE3) cells, which were grown at 30 °C in LB medium containing 30 μg mL−1 chloramphenicol; His-DnaK expression was induced by adding 0.1 mM isopropyl-1-thio-β-d-galactopyranoside (IPTG), followed by incubation at 30 °C for 3 h.
These plasmids were transformed into JPU and CEN.PK2 yeast cells which were grown in MD medium.
Cells, which were grown in serum, began to differentiate into capillaries at the 24-hr time point (Fig. 1D).
Sub-confluent BHK-21 cells, which were grown in six-well chambers, were infected with rabies virus strain CVS-11 at a multiplicity of infection of 0.1.
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Using animal cells, which are grown in enclosed steel tanks, also reduces the risk of bacterial contamination, which has led to shortages of seasonal vaccines in some years.
All but Jurkat cells (which are grown in suspension) were incubated at 37°C for 5 6 hr to allow for cell attachment, followed by addition of compounds via pin tool (Kalypsys, San Diego, CA).
In order to check whether there are differences in future metabolic profiles of the cells which are grown under stressful conditions some of the cells (experiments PC1 and PC2) were heat stressed during the final 40 minutes of growth prior to the in vivo NMR experiments (see also [ 4]).
As our control transfections resulted in viable colony formation in both A2780 and A2780DPP cells, we hypothesised that GSTP1 knockdown may be lethal in A2780DPP cells, which are grown in medium containing 1 μ M cisplatin to maintain the drug-resistant phenotype.
Only dilution is taken into account: KorA and KorB are relatively stable proteins and the data modelled come from exponential phase cells, which are growing rapidly.
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bacteria which were grown
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cells which were developed
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cells which were found
cells which were programmed
cells which were infected
cells which were designed
cells which was grown
cells which were created
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