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Lipid-containing cells were visualized by Oil-red O staining.
Antifungal drugs were added directly to the chambers and the cells were visualized by fluorescence microscopy beginning directly after addition of drugs and for the subsequent 30 minutes.
C. albicans strain Pma1-GFP were grown in YPD, and seeded onto microscope slides pre-coated with 1 mg/ml concanavalin A. Cells were visualized by fluorescence microscopy as above, using FITC filter (489 nm excitation, 511 nm emission).
Cells were visualized by immunofluorescence.
The transfected cells were visualized by fluorescence microscopy.
After 24 h, cells were visualized by light microscopy.
The cells were visualized by fluorescence microscopy at 100X magnification.
Ki67-positive cells were visualized by fluorescence microscopy.
The cells were visualized by fluorescence microscopy (Leica DM6000).
Invasive cells were visualized by staining with crystal violet.
Gr1+ cells were visualized by adding Alexa fluor-488 and Streptavidin-alexa fluor 488 (Invitrogen).
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