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Then, cells were transferred to LB plates containing kanamycin.
CD45.2+ T cells were transferred into CD45.1+ congenic mice, while CD45.1+ congenically labelled T cells were transferred into CD45.2+ WT mice.
Cells were transferred to 1.5 ml tubes and centrifuged at 20,000 × g for 20 min.
Differentiated cells were transferred to DMEM containing 2% horse serum upon reaching confluence.
The electroporated cells were transferred into a plate containing 2 ml TCM + IL-2 without antibiotics.
For serial transfer experiments, CFSE-labelled CD4+ T cells were transferred into mice for the primary transfer.
Cells were transferred to 96 well V-bottom plates (Thermo Scientific, UK) and centrifuged (300 × g, 5 min).
Following 6-month drug selection, HCC827 cells were transferred to erlotinib-free RPMI 1640 medium for a further incubation for 2 months (HCC827/ER cells).
Using a microdissection apparatus equipped microscope suitable for yeast (Singer Instruments), cells were transferred to defined places on the agar plates and virgin daughter cells were collected.
Cells were transferred to flow cytometry tubes and analysed by a BD LSRII or BD Fortessa flow cytometer for fluorescent analysis.
The culture tubes were vigorously vibrated with a vortex mixer for 3 s and non-adhesive cells were transferred to fresh tubes.
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