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A total of 1,000 cells were suspended in 100 μl Matrigel and overlaid in wells.
Cells were suspended at 2 × 106 cells/ml and mixed with the NPC solutions.
The harvested cells were suspended in TMA-I buffer and disrupted by sonication.
The cells were suspended in 500 μL of buffer and treated with 5 μL of Annexin V-FITC.
The sorted CD4+CD45RBhigh T cells were suspended in PBS and then kept on ice until required for use.
Cells were suspended in PBS containing 4% FBS and incubated with rat monoclonal antibody against CD31 (PECAM1) (BD Biosciences: 553370).
Collected cells were suspended in ice-cold 90% methanol and incubated at −30 °C overnight for fixation and permeabilization.
On EDD8, transfected PC3 cells were suspended in PBS and Matrigel (1 1) and implanted on the CAM.
After washing with PBS (0.3% FBS), the cells were suspended in the same buffer with propidium iodide (1 µg/mL).
These cells were suspended in a sea of neutrophils.
SGC-7901 cells were suspended in RPMI-1640.
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