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The adenylate energy charge (AEC) value mirrors the cellular energy status (Atkinson and Walton [1967]) and can be assessed as follows: Biocatalytic reactions inside the cells were stopped with 35% (w/v) HClO4.
P. abyssi cells were stopped at three different growth phases: exponential, end of exponential and stationary phases.
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At this pO2, the growth of E. coli cells was stopped and the cell pellet was collected by centrifugation at 6000 rpm for 20 min and stored at −20 °C.
The media flow over the cells is stopped periodically.
The migration of the cells was stopped 24 hrs later by exchanging the medium with 4% paraformaldehyde.
Incubation of the cells was stopped after 24 h, cell growth was examined with light microscopy and cells were fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich) for 20 minutes at room temperature.
The growth of the cells was stopped and cells were fixed overnight at 4° by adding 200 μL of ethanol.
This would mean that activated T cells are stopped by CTLA-4 from proliferating just to be eliminated by apoptosis, which would happen anyway by AICD.
After 21 days post infection (dPI), fresh media continued to be added to the culture every 3 4 days, but the addition of naïve 3201 cells was stopped and the % viability was allowed to decline (in the hope that a cell adapted virus could emerge that would enhance our ability to grow up viral stocks for use in Western blot assays).
The sequence of events during the calibration can be summarized in the following way (see the inset in Figure 8): (1) solution flow through the cell is stopped ("PUMP OFF"), (2) begin of illumination at 365 nm ("UV"), (3) change of wavelength to 410 nm ("VIS"), and (4) start of flow through sample compartment with next sample solution ("PUMP ON").
Cell cultures were stopped after 15 passages.
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