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For immunofluorescence microscopy bacterial cells were spread on a glass coverslip coated with 0.1% poly-L-lysine, air-dried and fixed in 2% paraformaldehyde.
As we recently described using comparable experiments5, β1-integrin is shown to retain a similar spatial pattern similar to zyxin when the cells were spread on the substrate, in their pre-division shape (Fig. 2d).
Following incubation, appropriate dilutions of treated and untreated cells were spread on BHI agar plates.
Cells were spread on LB agar containing the appropriate antibiotics and incubated at 30 °C or 37 °C.
The transformed cells were spread on LB agar plates and, after antibiotic selection and blue/white staining, colonies were selected for colony PCR and direct sequencing.
The transformed E. coli cells were spread on LB agar plates containing 50 μg/mL ampicillin and 34 μg/mL chloramphenicol, and then cultured overnight at 37 °C.
After adding Propidium iodide the cells were spread on the glass slide and covered by cover slip and observed by fluorescent microscope.
The PHB-producing cells were spread on the agar plate containing LipidGreen1 at a final concentration of 25 µg/mL and cultured for 20 h at 37°C.
The suspended cells were spread on plates containing SD/-His/-Leu/-Trp medindicatedied with indiconcentrationratiof of 3-AT (3-amino-1, 2, 4-triazole) and ABA.
When cells were spread on the selective plate containing 0.02 mmol/L IPTG and subtracting tryptophan for both Rubisco and PRK expression, wildtype Rubisco exhibited normal cell growth, while the inactive RbcL197 mutant still suffered the growth arrest (Fig. 3E), suggesting that it was indeed the Rubisco activity that rescued the cell.
Untreated cells were spread on selective SC plates as control.
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