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After electroporation recombinant cells were selected on medium containing chloramphenicol.

Transformed cells were selected on LB agar with ampicillin, and the plasmid was extracted using a plasmid DNA purification kit (NucleoSpin Plasmid, Macherey-Nagel).

After transformation of a- and α-type derivative cells with the plasmids pLY-hygro and pHY-kan, cells were selected on YPD + HYG and YPD + G418 plates, respectively.

Hybrid a/α-type cells were selected on solid YPD medium containing HYG and G418 (YPD + HYG, G418 plates) and were then isolated on YPD plates after the removal of both plasmids through passage cultures performed with YPD medium without antibiotics.

Cells were selected on SC-trp-ura plates.

Transformed cells were selected on SC-leucin plates.

It was proposed that if the repertoire of antiviral CD8+ T cells was selected on constitutively immunoproteasome-rich professional APCs, such as DCs and macrophages, they might predominantly be specific for epitopes that are better presented by the immunoproteasome [30].

Because the mechanism of positive selection occurs at the double positive stage (35), we favor the possibility that all T cells are selected on the VP2 antigen and are deleted from the active repertoire independent of CD4 or CD8 expression.

Sf9 cells were selected based on their demonstrated success in other GPCR structural studies.

For analysis of c-Fos expression, transfected cells were selected based on the RFP signal.

Differentiation induces Olig2 expression and differentiated cells were selected based on labeling with Olig2 antibodies.