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Cells were refrigerated in an ice bath, treated with a second round of glass bead extraction, pelleted by centrifugation at 14 000 r.p.m. and tested for β-galactosidase after 10 min at 37 °C.
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"All those plants were refrigerated by ammonia pipes," he said.
All the samples were refrigerated until further use.
Samples were refrigerated at –20°C.
Samples were refrigerated immediately upon collection.
Aliquots of baseline maternal serum were refrigerated.
All samples were refrigerated at 4°C.
Samples were refrigerated immediately after processing until shipment.
The milk samples were refrigerated immediately after sampling.
Final purified extracts were refrigerated at +4°C until use.
The carcass samples were refrigerated 24 hours after pre-cooling.
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