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Individual cells were planted on the surface of solid medium by micromanipulation, and the efficiency of zygote formation in the four grandchildren of a cell was recorded.
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HeLa cells stably expressing EGFP or Calu-15 EGFP Calu-15 EGFP on coverslips at the same density.
Both plants were planted on 3 October 2015.
Senior German intelligence officials say they have no real idea how many terrorist sleeper cells could be planted on German soil, like the Arab men in Hamburg who used the efficiency, easy travel, good communications and multiethnic character of a large German city to plan their attacks.
The RPE suspension was planted on 24-well cell culture plates at 5% (v/v) CO2/37°C.
Extracellular fibrils were also formed when C. gattii cells were grown on plant-based substrates, and on materials promoting cell adhesion.
Transformed cells were recovered, and plants regenerated on selection media, propagated and sampled for DNA, RNA and protein analysis.
Cells were cultured on coverslips.
The cells planted on coverslips were fixed with 4%% paraformaldehyde at room temperature for 15 min, permeabilized in 0.1 % Triton X-100 for 15 min, blocked by 5 % BSA at 37 °C for 30 min, incubated with primary antibodies at 37 °C for 2 h, and stained with Alexa Fluor 488-conjugated or 568-conjugated IgG at 37 °C for 1 h.
Possible toxic effects of multi-walled carbon nanotubes (MWCNTs) on plant cells were investigated.
Altered patterns of protein glycosylation in transformed cells were initially exploited using plant lectins based on their specific binding to distinct sugar sequences [ 13].
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