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Three cells were planted with Typha domingensis Pers., and three cells were left unplanted.
Briefly, the cells were planted into the dishes using RPMI 1640 medium supplemented with 2 mmol/L L-glutamine, 10 mmol/L HEPES, 20 µmol/L 2-mercaptoethanol, 150 U/mL streptomycin, 200 U/mL penicillin, 10% FBS and stimulated with combinations of GM-CSF (40 ng/mL) and IL-6 (40 ng/mL).
For transduction, RAW264.7 cells were planted into six-well tissue culture plates at 2.5×105 per well.
Infected cells were planted into tissue culture flasks and incubated for 48 hours (≥90% infected and 100 400 rickettsiae per cell) before harvesting.
MIN-6 β cells were planted in the bioscaffold.
Forty-eight hrs later, 200 cells were planted in a six-well plate.
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Wild-type HGC27 and AGS cell were planted under concentration gradients starting from five times blood concentration.
HCT-116 cells (3000 per well) were planted into 96-well culture plate.
Republican Senator James Risch of Idaho said he found the news "very troubling," and wondered whether the men formed part of a militant sleeper cell to be planted in the United States, or whether it was something far more innocent.
Equal number of labelled cells were mixed and planted in 6 cm culture dishes.
Cells were exposed to plant extract, Ag and Au NPs over a range of concentration for 24 h.
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CEO of Professional Science Editing for Scientists @ prosciediting.com