Sentence examples for cells were phenotyped from inspiring English sources
Cells were phenotyped and subjected to automated processing and activation using TransAct, a polymeric nanomatrix activation reagent incorporating CD3/CD28-specific antibodies.
SIV+ cells were phenotyped.
Cells were phenotyped by three-colour flow cytometry (FACSaria) according to the expression of CD25 and Foxp3 using the mouse regulatory T cell staining kit from Ebiosciences, UK.
After 5 days of culture 100 µL of supernatant was collected and stored at −20°C to quantify the production of cytokines, and the remaining cells were phenotyped by flow cytometry.
Cells were phenotyped using the following antibodies and run on the BD FACSCalibur (BD Biosciences): CD3-APC clone SP34-2 (Biosciencesces), CD8ß-PE clone 2ST8.5H7 (Beclone Coulter); CD3-APC (BD Biosciences), CD8α-PerCP clone SK1 (BD Biosciences); CD3-APC (BD Biosciences), CD4-PerCP clone SK3 (BD Biosciences).
T cells were phenotyped according to manufacturer's instructions, fixed in 150 µL fix buffer (2% formalin in PBS) and processed on the 4 parameter BD FACS Calibur™ flow cytometer using the following antibodies: CD4 PE (SK3), CD4 APC (SK3), CD8 PerCP (SK1), CD25 FITC (M-A251), IL-10 PE (RM4-5), Ki-67 FITC (B56/MOPC-21 B56/MOPC-21ngen, France) and FOXP3 APC (PCH101) (e-BD PharmingenS).
To illustrate class specific patterns could be established, the same cell lines were phenotyped for sensitivity to the Topoisomerase 2 (Top2) inhibitors, etoposide and teniposide.
T cell lines were phenotyped for 24 TCR Vβ expression (IOTest Beta Mark PN IM3497) and anti-CD3-PE-Cy5 mAb (Beckman Coulter).
Based on these findings EpCAMhigh and EpCAMlow breast cancer cell lines were phenotyped for markers of epithelial or mesenchymal differentiation.
A total of randomly selected 50 BrdU-positive cells per animal were phenotyped.
The cells were then phenotyped by flow cytometry.
