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In contrast, despite being able to initiate a tumor, CD95neg and unsorted tumor cells were not able to sustain tumor growth.
However, the adherent cells were not able to sustain growth over time.
Fermentation analysis in bioreactors showed that, except for the reference strain (CN1), cells were not able to grow under strict anaerobic conditions.
It may surprise but solubilized morphogen molecules in form of a supernatant from Wnt4 expressing cells were not able to induce formation of tubules in isolated nephrogenic mesenchyme.
Chimera analysis showed Mesp1/Mesp2 double-knockout cells were not able to undergo epithelialization, whereas Mesp2 single-knockout cells were integrated into epithelial somites and dermomyotome occasionally (Takahashi et al., 2005).
Similarly the fadJ gene which is a part of the anaerobic β-oxidation of fatty acids was also down-regulated suggesting that the cells were not able to use fatty acids as carbon and energy source (Campbell et al. 2003).
In our hands, ESP cells were not able to proliferate from a single cell in vitro.
The remaining MOG-specific T cells were not able to proliferate when stimulated in vivo by MOG-presenting APCs.
More importantly, PTEN-deficient Caco-2/15 cells were not able to form metastases when injected into the tail vein.
However these cells were not able to progress towards leptotene stage compared to the Kit positive cells collected at 7dpp.
In addition, centrioles from CDK11-depleted cells were not able to over duplicate even if forced Plk4 overexpression was induced.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com