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Mean fluorescence intensities (MFIs) of CHC and Cav-1 siRNA treated cells were normalized to MFIs of cells transfected with non-silencing siRNA.
To analyze BST-2 transcript levels, all human samples were normalized to HMLE cells and murine cells were normalized to E0771 shControl (shCTL) cells.
In all cases, the levels of pre-mRNA before any treatment in both WT and KD cells were normalized to 1 and the pre-mRNA levels at each time point was relativized to this level.
(a) Mean (±SEM) levels of VGLUT1 mRNA in stably transduced cells were normalized to expression of the acidic ribosomal protein 36B4, and expressed as a percentage of those following control nontargeting shRNA.
Background was substracted from raw data and infected cells were normalized to 100%.
For data analysis total annexin V-PE positive cells were normalized to transfection efficiency.
Similar(25)
Signal observed in untreated host cells represents the background detection of the probe; for parasite-derived miRNA, the data are normalized to the input detection level of miRNAs in 5 μg of exosomes, whereas miR-16 levels in exosome-treated cells are normalized to untreated cells.
In the experiments presented here, miRNA expression in cells was normalized to U6 snRNA, as is common in many other reports53.
The relative level of GFP expressing cells was normalized to cells from wildtype littermates.
The number of cells was normalized to rat BW for data analysis.
For example, data from treated cells are normalized to untreated cells, according to the formula: 2−ΔΔCt = 2−ΔCt treated cells)/2−ΔCt untreated cells).
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