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When the stem cells were manipulated in animals with TMJ degeneration, the cells repaired cartilage in the joint.
This approach allowed us to fix the proteins with formaldehyde while the cells were still in the culture dish, locking in the results before the cells were manipulated in any way.
After the culture reached steady-state, the cells were manipulated in two ways: for enzyme assays, flux measurements and nucleotide determinations, the flows were stopped and methanol was added to the succinate-limited steady-state culture to a final concentration of 50 mM.
Shortly thereafter biological cells were manipulated using infrared lasers [ 5].
For this manuscript, the D562 cells were manipulated before reaching 100% confluence.
Cells were manipulated to generate: G0/G1-, S-, and G2/M-enriched cultures.
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Those brain cells were George Balanchine's, and therein lay the solution to the mystery of his death last year at 79 years of age.
Inside the prisons, the cells are Spartan.
EPO (a.k.a. erythropoietin), a naturally occurring hormone that increases the production of red blood cells, was Edgar — short for Edgar Allan Poe.
Viable cells are Annexin V negative and 7-amino-actinomycin D (7AAD) negative.
Cells in the early phases of cell death are Annexin V positive and 7AAD-negative, and necrotic cells are Annexin V positive and 7AAD-positive (Figure 2A).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com