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Briefly, cells were lysed using glass beads in 20% TCA buffer.
Cells were lysed using 300 mM FA lysis buffer (containing protease inhibitor cocktail).
Red blood cells were lysed using ammonium chloride solution (STEMcell Technologies).
After removing the filters from the liquid, cells were lysed using a sonicating probe3.
Subsequently, cells were lysed using liquid nitrogen and two repeated cycles of freeze-thaw.
Red blood cells were lysed using Ammonium Chloride Potassium Lysing Buffer.
Cells were lysed using RIPA buffer (Thermo Fisher Scientific) supplied with Halt Protease and Phosphates Inhibitor Cocktail (Thermo Fisher Scientific) for Western blotting.
After treatment with inducers, cells were lysed using RIPA buffer and total protein was used to analyse the expression of AhR, Cyp1A1 and Cldn4 by western blot.
At each time point, cells were lysed using TRIzol® reagent (Thermo Fisher Scientific) and total RNA extraction performed using the phenol/chloroform extraction method.
Cells were lysed using cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630).
Cells were lysed using RIPA buffer supplemented with protease inhibitors.
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