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Transfected cells were incubated in a normoxic incubator for 24 h following siRNA transfection.
Control cells were incubated in medium only.
Cells were incubated 1 hr at room temperature.
Cells were incubated with BDNF and cycloheximide (CHX).
Next, cells were incubated with 10% Cell Counting Kit-8.
After the irradiation, cells were incubated for 24 h.
Then, the cells were incubated for an additional 18 h.
After 24 h, the cells were incubated with different amounts of KH176(m) for 24 h.
Cells were incubated at 37 °C in 5% CO2 for 24 h.
Cells were incubated for 4 hours at 37 °C protected from light.
After 16 h serum starvation, cells were incubated with treatment medium (as described above).
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