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Three units of packed cells were given, with the hemoglobin concentration rising from 7.2 to 9.5 mg%.
The first step in the process was to run the model in "cold-start" mode, where in the first year of the simulation all forest cells were given C stocks of zero, signifying a forest age of zero.
After 24 hours, the cells were given fresh medium and harvested after an additional 24 hours.
These cells were given a 1∶2 split ratio while passaging these as differentiated endothelial cells.
Bromodeoxyuridine (BrdU) injections to evaluate survival of proliferating cells were given on days 21 and 22 of fluoxetine treatment.
The cells were given pulses of the cortisol medium for 10 minutes followed by a 50 minutes washout period.
Cells were given 3 h to coalesce and then a remaining 400 µl of medium was added to each well.
Cells were given several hours to recover post-bombardment before embedded nanoparticles were tracked with high spatial and temporal resolutions using high-magnification fluorescence microscopy (60×).
To create complete spatial coverage, missing data for some grid cells were given the 1961 1990 mean climate values for certain time periods.
At 70% to 80% subconfluency, the cells were given fresh 10% FBS/DMEM media 24 hours before inoculation into the mice.
Non-rounded cells having three or four membrane extension processes and with a cell morphology similar to wildtype HEK-293 cells, were given zero points.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com