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In these mice, CD4+ T cells were generated with the appropriate maturation phenotype and showed a diverse repertoire of TCR Vβs.

The resulting CD36+ cells were generated with a defined combination of growth factors [7].

After retransfer of pWRG99 in WRG38, electrocompetent cells were generated with λ Red recombinase induced by addition of 10 mM L--arabinose.

KChIP.1 up-regulation appeared to be specific for central memory T cell differentiation since no up-regulation occurred when memory T cells were generated with low dose IL-2, except a small isolated increase on day 6 (Fig. 5,A and B), whose significance remains unclear.

CadN R7 cells were generated with MARCM, using GMR-FLP and positively labeled with CD4-tdGFP.

Shortly after that, human iPS cells were generated with similar strategy.

Cell colonies (or spheroids), with an estimated 103 cells, are generated with the hanging droplet method [36] and subsequently seeded in 500 µL of collagen solution at 1.5 mg/mL shortly before it polymerizes.

A gridded surface of 5 km × 5 km cells was generated with each pixel representing either urban, peri-urban, or rural areas.

CM for testing with NK cells was generated with 72-h cultures of monocytes (10/ml), CHLA-255-Fluc neuroblastoma cells (10/ml), or CHLA-255-Fluc/monocytes together (10/ml of each cell type) in IMDM and 15 % FBS.

The methylome of human H1 embryo stem cell was generated with Illumina single-end (75 bp in length) sequencing strategy and the methylomes of female adipose-derived stem cells (ADS), adipocytes derived from the ADS cells (ADS-adipose) and ADS induced pluripotent stem cells (ADS-iPSCs) were generated with a paired-end (75 bp x 2) bisulfite sequencing approach [ 5, 30].

Stable transfectant cells were generated by selecting with G418 at a concentration of 300 µg/ml.