Sentence examples for cells were frozen down from inspiring English sources
On the same time we strongly suggest the further use of the same cell lines been transfected in this studies in Orthotopic Xenotransplantation Studies, as we best stable cells were frozen down already.
Cells were frozen down in multiple aliquots at passage 3 to 7, and stored in liquid nitrogen until use.
When cultures reached confulence cells were frozen down in liquid nitrogen until further use.
Initially, cells were frozen down after every passage, however after passage 20 this was reduced to every five passages.
Cells were washed in water and pellets of 1 2 × 10 cells were frozen down in liquid nitrogen and stored at 80°C until analysed.
After mating, cells were frozen down until further processing (ethanol tolerance assay for genome shuffling with selection or pre-growth before sporulation for genome shuffling without selection, see also Additional file 1: Supplemental Text).
Every time a batch of cells is frozen down, it is recommended that one vial is resuscitated immediately to check viability.
After separating the cells from the culture broth, cells were frozen at −30°C and freeze dried.
Cells were frozen from the 5th passage.
