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Those cells were frozen and shipped to Dr. Prather in Missouri.
A95 cells were frozen in liquid nitrogen and ground with a mortar and pestle.
After separating the cells from the culture broth, cells were frozen at −30°C and freeze dried.
Cells were frozen from the 5th passage.
The cells were frozen and homogenized with a mortar and pestle under liquid nitrogen.
Cells were frozen, kept in liquid nitrogen and thawn on demand.
Isolated cells were frozen at −80°C in R10 with 10% DMSO for flow cytometric analysis.
Aliquots of cells were frozen at each passage and stored in liquid nitrogen for future studies.
The cells were frozen at −20°C and kept frozen for at least 4 days.
The cells were frozen in liquid nitrogen as small pellets and stored at −80°C.
When the CPE reached 90%, the culture cells were frozen and thawed three times.
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