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Cells were freeze dried at −40°C and 5 mbar pressure and viability was measured before and after freeze drying on BSM agar.
Content of adenosine phosphates Pelleted HL-1 cells were freeze dried then extracted with 1.3 mol/l perchloric acid and neutralised with 1 mol/l KHCO3.
The cells were freeze thawed once, resuspended in 0.25 M sucrose, 10 mM Tris/base pH 7.2, 2 mM EDTA and 0.1 mM PMSF (Sigma) and homogenized using a tight-fit glass-glass homogenizer.
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Those cells were frozen and shipped to Dr. Prather in Missouri.
The cells were freeze-thawed once, then disrupted with sonication on ice followed by centrifugation.
For Se analysis culture samples (50 mL) were centrifuged and sediments of cells were freeze-dried before analysis.
After separating the cells from the culture broth, cells were frozen at −30°C and freeze dried.
A95 cells were frozen in liquid nitrogen and ground with a mortar and pestle.
Finally, the harvested cells were frozen at -20°C overnight and the frozen cells were then subjected to lyophilization for 48 hours by using a Labconco 4.5 Freezone apparatus.
For EMCV and HSV-1, infected cells were freeze-thawed, and the supernatants were used.
Cells were freeze-thawed three times and insoluble material was pelleted.
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