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Tibia and femur were extracted aseptically and the bone marrow (BM) cells were flushed from tibia and femur of mice.
Bone marrow cells were flushed out of the femurs and tibias of 4-week-old green fluorescent protein-transgenic Wistar rats.
Bone marrow cells were flushed and isolated as previously described.
Cells were flushed out using sterile PBS and collected in 15 ml Falcon tubes.
After three washes, the column was removed from magnetic filed and CD11b+ cells were flushed out.
In brief, bone marrow cells were flushed out of femur and tibia bones with PBS.
Briefly, bone marrow progenitor cells were flushed out of the bone marrow using ice-cold RPMI medium.
Bone marrow cells were flushed from the bones using a 26 g needle until the bone is clear.
Bone marrow monocyte/macrophage (BMM) precursor cells were flushed out from femurs and tibias by 25-G needle.
In brief, cells were flushed out of leuko-reduction filters with 40 ml PBS without Ca2+ and Mg2+ using a syringe.
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The cells with magnetic corpuscles bound on the surface are kept in the field while non-stained cells are flushed out from the bag.
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CEO of Professional Science Editing for Scientists @ prosciediting.com