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After washing cells were fixed for 15 mins.
Migrated cells were fixed for 15 min in 4% paraformaldehyde.
One week later, cells were fixed for immunostaining.
AP-activity was measured and adherent cells were fixed for SRB staining of surface proteins.
Briefly, cells were fixed for at least 30 min in 95% ethanol.
Cells were fixed for 10 minutes with 2% (w/v) formaldehyde in PBS.
Cells were fixed for 30 minutes in a 4% parafomaldehyde solution in PBS.
Similar(4)
Cells were fixed for 1 h at 4 °C in phosphate buffer (PB) with 2.5% glutaraldehyde.
Briefly, HeLa cells were fixed for 20 minutes in warm PBS with 4% paraformaldehyde and permeabilized with 0.5% saponin.
After drying the cells at room temperature, cells were fixed for 10 min with PBS containing 4% paraformaldehyde.
After 48 h, transfection cells were fixed for the purpose of immunofluorescence labelling.
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