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Next the flow cells were filled with a buffer and the buffer was pumped through the cells at a slow and constant flow of 100 μL/min.
Recorded cells were filled with neurobiotin present in the intracellular solution via positive current injection (400 pA for 250 ms, 1.25 Hz) for at least 6 min. After recording, coverslips were fixed in 4% PFA 0.01 mol/L PBS for 10 min then rinsed with 0.01 mol/L PBS.
On the fifth floor, cells were filled to capacity and bunks were squeezed into the common dining area to handle the overflow.
Prior to inoculation the cells were filled with control solution.
During recordings, all cells were filled with biocytin, and subsequently processed for the immunohistochemical presence of TH (Fig. 3A).
In contrast, areas with no blood cells were filled with laminin-containing ECM (small bracket in Fig. 4E and 4F).
Similar(24)
It maintains its shape whether the gas cells are filled or empty.
The Cornalbo Dam features masonry walls that form cells; these cells are filled with stones or clay and faced with mortar.
In some hibernating bats the granulosa cells are filled with glycogen, or animal starch, which may be a source of energy.
The space between cells is filled by a fluid that may flow freely within this extracellular space.
Cells are filled with biosensors, molecular systems that measure the state of the cell and respond by regulating host processes.
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