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The cells were fed with carbon fuel obtained by the direct RF plasma splitting of methane.
Then, the cells were fed once at the interval of 1 day with the identical dose of rmGM-CSF plus rmIL-4 for 6 days.
Cells were fed and fixed as above.
Cells were fed every 2 days.
Cells were fed twice a week for four weeks.
From day 3 forward, cells were fed 100% NM.
Cells were fed every 4 days and colonies were counted microscopically after 4 weeks.
Cells were fed weekly with growth media and plates were examined daily for 3 weeks.
Cells were fed daily and passaged when they reached 70 80% confluency.
Cells were fed with DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin.
Cells were fed every three days with the same fresh culture medium.
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