Cells were extracted in M-per mammalian extraction reagent (Pierce) with Complete (Roche) protease inhibitors.
Using rapid, chilled chemomechanical disruption, the neuron cells were extracted and purified, then sorted using fluorescence-activated cell sorting (FACS).
Her T cells were extracted in preparation for the trial, even as she got sicker and sicker.
Cells were extracted after 2 days or were subjected to serum starvation for 1 day before treatment with various inhibitors.
After 7 d, cells were extracted from the ascites, washed with D-Hank's solution, and centrifuged at 500×g for 5 min at 4 °C (5 cycles).
Nuclei from control and KD NMuMG cells were extracted, and soluble and loosely bound chromatin proteins were separated from the insoluble chromatin fraction.
Cells were extracted from the outer and inner part of eight menisci of four 2-year-old merino sheep.
Some descriptors based on boundary and area shapes of Voronoi cells were extracted for each fiber distribution.
NK cells were extracted by EasySep™ human NK cell enrichment Kit (STEMCELL Technologies, Inc., Vancouver, Canada).
Afterward, RNA samples from the different cells were extracted following the protocol of QIAamo® RNA Blood Mini Kit from QIAGEN.
