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The NPC primary culture cells were expansion in vitro for 5 to 8 generations and then frozen for later use.
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Further the cells were quantitated for expansion and apoptosis by various methods.
After colonies of spindle-shaped cells formed, the cells were reseeded for expansion.
Cells were cultivated in expansion media containing either aSera or ySera.
On day 5 after admission, peripheral blood mononuclear cells were examined for expansion of Vβ2 T cells.
For subsequent cell expansion, cells were grown in control medium at 37 °C maintained at 5% CO2 and 5% O2.
For cell line expansion, cells were trypsinized at 80% of confluency.
After expansion, cells were sorted (MoFlo cell sorter, Beckman Coulter Inc).
Following expansion, cells were differentiated according to a trichostatin-A/GLP protocol.
Following expansion cells were seeded in 6-well culture plates (50 cells/cm2).
Following the 10-day drug selection, single isolated cells were chosen randomly for expansion.
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