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Pooled Cas9 cells were expanded under blasticidin selection up to their transduction with the CRISPR libraries.
Furthermore, these mutation-bearing cells were expanded in number at the time of relapse (Supplementary Fig. 4A).
Single cells were expanded into clones and on-target effects were validated through Sanger sequencing following the previously described protocol26.
Cells were expanded in NES medium and reached confluency (0.5 1 × 105 cells/cm2) in about 7 days.
Cells were expanded to passage 4, whereupon they were used for experiments.
UCB cells were expanded in the device, then administered as a boost to the conventional graft on posttransplantation day 12.
Prior to the start of the in vitro experiment, W20 17 cells were expanded and cryopreserved in multiple aliquots.
Cells were expanded and then infused into the patients.
Subsequently, transfected cells were expanded.
The cells were expanded and continuously grown under selection.
LNCaP and HUVEC cells were expanded separately in T75 flasks.
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