Human NK cells were enriched from Buffy-coats (EasySep negative enrichment kit, Stemcell Technologies, 19055).
Target cells were enriched using magnetic microbeads and an autoMACS® Pro Separator (Miltenyi Biotec).
Transduction efficiencies were scored by fluorescence microscopy, and transduced cells were enriched by fluorescence-activated cell sorting (FACS).
Asterisks indicate cells were enriched for oligodendrocyte-lineage. Brain model schematic obtained from GENSAT (Gene Expression Nervous System Atlas) [120, 125].
Comparison of differentially expressed genes between wild type and knockout cells, we found that genes up-regulated in the wild type cells were enriched for the terpenoid backbone biosynthesis pathway, whereas genes down-regulated in the wild type cells were enriched for the focal adhesion and vascular smooth muscle contraction pathways (Table 1).
Patient peripheral blood lymphocytes were coelectroporated with SB11 transposase and Sleeping Beauty transposon, and transposed T cells were enriched by sorting on murine TCRβ (mTCRβ) expression.
The transfected cells were enriched by flow sorting (Figure 1C).
The transfected green cells were enriched by selection with puromycin.
eGFP-expressing cells were enriched by cell sorting.
Cells were enriched using 2 successive columns to increase purity.
Epithelial cancer cells were enriched by growing cells in sphere culture conditions.
