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The cells were embedded in EmBed 812 DER 736.
After dehydration in a graded series of acetone, the cells were embedded in Embed 812 epoxy resin.
After centrifugation, pelleted cells were embedded in 12% gelatin in 0.1 M PB.
Using increasing concentrations of ethanol followed by propylene oxide, the cells were embedded in epon resin.
Afterwards, the cells were embedded in Mowiol 4 88 and visualized using an Olympus IX70 fluorescence microscope.
Hence, we performed a series of 3D clonogenic spheroid assays in which cells were embedded within matrix over time.
Spheroid formation was observed exclusively in 3D when cells were embedded within hydrogels.
Single cells were embedded in Matrigel and seeded in 24-well plates (15,000 single cells per 50 μl Matrigel per well).
Membrane proteins derived from PMA-stimulated U937 cells were embedded into liposomes containing alkaline phosphatase and reacted with the indicated doses of purified uPA immobilized on Sepharose 4B.
E. coli or B. subtilis cells were embedded in agarose gel plugs at a concentration of 5 × 108 cells ml−1.
After the cells were embedded in 3D collagen matrices with different collagen concentrations, they were stimulated with TGF-β for seven days.
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