In contrast, K14-positive cells from human iPS cells were dispersed among K18-positive colonies.
Detached cells were dispersed in 4 mL of complete growth medium and gently pipette out of the well.
The cells were dispersed in 35 mm dishes coated with type I collagen (Iwaki, Chiba, Japan) with D-MEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Gibco) and a 1% mixture of penicillin, streptomycin, and neomycin (Gibco).
After, the cells were washed with PBS (0.1 M, pH 7.4) three times and dispersed in PBS, and then the cells were dispersed with 350 μL of 1% agarose gel per sample for MRI with 3.0-T whole-body MR scanner.
Cells were dispersed using an 18-gauge needle, pelleted, and resuspended in 5 ml HBSS.
ES cells were dispersed into single cells; the cells were fixed overnight in 70% ethanol at 4°C.
For the intracranial implantation experiments, U87-MG cells were dispersed with a 0.05% solution of trypsin/EDTA (Life Technologies), reaction was stopped with FBS.
All of the cells were dispersed in the fresh PBS and mixed with the antibody, anti-IL13RA2, the concentration of which was 25 µm ml−1.
In adherent differentiation ES cells were dispersed with 5 mg/ml dispase and plated onto gelatin-coated 6-well dishes (Becton Dickinson, Franklin Lakes, NJ, USA).
In methocel treated mice, dark cells were dispersed among intact neurons in the striatum and the motor cortex (Fig. 5B,D,H,K).
Most of these cells were scattered deep within the folds of the villi, however, some of the cells were dispersed along the apical regions of the villi.
