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The cells were determined using a hemocytometer under a light microscope (Nikon Optiphot, Japan).
Twenty-four hours later, viable cells were determined using the MTT assay.
LaF3 Tb nanoparticles assessed in 3T3 cells were determined using various concentrations by use of the WST-1 assay.
The survival fractions of treated cells were determined using the ratio of the number of colonies formed/number of cells seeded × plating efficiency.
The effects of EAF of T. conoides on apoptosis of HepG2 cells were determined using annexin V-FITC and PI staining.
Total cell count and the rate of unviable cells were determined using a haematocytometer.
Optimal transfection conditions for G401 F22 cells were determined using BLOCK-iT fluorescent oligo (Invitrogen).
The apoptotic cells were determined using a Becton-Dickinson FACScan cytoflurometer (Mansfield, MA, USA).
The cell cycle distribution and percentage of apoptotic cells were determined using a FACS calibur flow cytometer (Becton Dickinson, USA).
ATP levels in dissociated brain cells were determined using the bioluminescent measurement of ATP as published [45].
Proportions of resting, proliferating and hypertrophic cells were determined using Openlab 4.0.4 software (Improvision) from at least three different mice.
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