Sentence examples for cells were cryopreserved from inspiring English sources

The remaining cells were cryopreserved in cryopreservation medium composed of dimethylsulfoxide (Sigma Aldrich) (10% in FBS).

The remaining cells were cryopreserved in cryopreservation media (10% dimethyl sulfoxide and 90% FBS), frozen at −80°C in an isopropanol jacketed closed container (Nalgene Cryo freezing container), and stored in liquid nitrogen until the next day.

Thus, the cells were cryopreserved from 4th to 6th passages.

Half of the cells were cryopreserved for 3 months.

Cells were cryopreserved for 24 h and cell viability post-freezing and thawing was quantified by trypan blue exclusion assay.

The T cells were cryopreserved in a solution of 90% fetal calf serum and 10% dimethylsulfoxide (DMSO) at 1 × 108 cells/vial.

A fraction of the cells was cryopreserved as described previously [ 13].

An aliquot of a mixture of cells was cryopreserved in 10% dimethyl sulfoxide (DMSO) containing FCS at −70°C.

After isolation, cells are cryopreserved in RPMI 1640 containing 20% of heat-inactivated foetal calf serum (FCS) and 10% dimethyl sulfoxyde (DMSO).

A portion of the recovered CD4+ T cells was cryopreserved for later use, and the remainders were depleted of CD45RO+ cells using anti-CD45RO-coupled magnetic beads and LD negative selection columns (Miltenyi Biotec).

Control was prepared by culturing approximately one-third of PBMCs without PA2024; the remainder of the cells was cryopreserved for possible use following disease progression to manufacture a salvage treatment manufactured according to the same specifications as sipuleucel-T.