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The National Institutes of Health has proposed new guidelines to allow scientists receiving its money to work with the cells, provided that the cells are created with private money.
The heat map for the drug-treated cells was created with the software GENE-E (Broad Institute).
The first embryonic stem cells were created at the University of Wisconsin with Geron's money, and the company spent more to buy intellectual property in cloning, which is expected to be important in some stem-cell work.
HEK293-LBD cells were created by transfecting HEK293 with pBD-LBD and selecting for Neomycine-resistant transformants.
These solar cells were created by combining liquid-phase epitaxy with gas-phase zinc diffusion technologies.
Mouse septal neuron x neuroblastoma hybrids SN56 cells were created by fusing N18TG2 neuroblastoma cells with murine (strain C57BL/6) neurons from postnatal 21 days septa [19].
UTRM10-676A and -WT cells were created by transfecting UTRM10 cells (Effectene, Qiagen) with LAP-Mps1-T676A LAP-Mps1-T676A LAP-Mps1-T676Aivors by continuous culture in 1 µg·ml−1 doxycycline (Sigma).
These cells were created by transfecting UM-SCC-5 cells with a pBABE-U6 vector containing STAT-3 short hairpin RNA (shRNA) as previously described [ 6].
Expression constructs, siRNA sequences, and antibodies are described in detail in Supplementary file 1. Constructs for expressing wild type and mutant NEDD4L and AMOT proteins in E. coli, insect SF-9 cells, and human 293T cells were created by standard cloning and mutagenesis methods, with detailed methods available upon request.
A dilution series of K562 cells was created by combining them with HCT116 colon cancer cells without the BCR- ABL1 translocation.
But these cells, which were created with funding from Menlo Park, Calif.-based Geron, aren't guaranteed to work.
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