Each cell measured eight feet by six feet, and the bars on the front of some cells were covered with Plexiglas.
In this instance, however, the holes in the men's cells were covered by revealing photographs of women in bikinis, which officials displayed at Monday's news conference.
Cells were covered with 2% ESC qualified-Matrigel diluted in N2B27 medium at day 7.
Post-fixation, the cells were covered with 1% osmium tetroxide (Electron Microscopy Sciences, USA) solution for 30 min.
The chips with attached bacterial cells were covered with 2.5% glutaraldehyde and kept for 3 h in 4 °C after which they were washed thrice with the phosphate buffer solution.
The original technique required injecting cells into the chondral defect, and these cells were covered with a periosteal patch that, due to its chondrogenic potential, allowed tissue repair with histological characteristics similar to the hyaline cartilage [2, 7, 8].
The treated and untreated cells were covered with a plate sealer then allowed to grow and proliferate by further incubation the plate for 24 h at 37 °C in humidified atmosphere of 5% CO2.
Attached aggregates of cells were covered with 2% ESC qualified-Matrigel diluted in N2B27 medium consisting of DMEM/F-12 supplemented with 0.1 mmol/L non-essential amino acids, 1 mmol/L GlutaMAX, 1% N2 supplement (Invitrogen) and 2% B27 (Invitrogen).
Transmission electron micrographs of the mixed co-cultures on day 14 showed that the mesenchymal cells were covered by an epithelial monolayer, and some of the epithelial cells had lamellar body formation (Fig. 7a), which was similar to that of freshly isolated type II AECs (Fig. 7b).
Cells were covered and kept on ice until analyzed on a FACSCalibur flow cytometer.
After a final wash, the cells were covered with a coverslip and mounting medium.
