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With regular intervals both the full-scale RO membrane modules and the flow cells were cleaned using conventional chemical treatment.
After that, the cells were cleaned twice with DPBS and incubated with 100 μL of 0.5 mg/mL MTT for 2 h at 37 °C testing for cell viability.
After 72 h, the cell supernatant was removed and the cells were cleaned with PBS twice, then kept in PBS/ethanol (1 1) for 2 min and fixed with cold ethanol for 10 min.
The supernatant was removed, and cells were cleaned and re-spun with PBS (1X, pH: 7.2).
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The release cell was cleaned with deionized water after each use.
After every experiment, ED cell was cleaned with circulation of 0.1 M HCl solution during 15 min to remove any deposits, followed by circulation of distilled water.
Between measurements of the different solutions the optical cell was cleaned thoroughly with Milli-Q water and rinsed with a small amount of the sample of the new solution from which absorbance data was to be collected.
During the determination of the conductivity of the different Casiopeína III-ia solutions the samples were stirred and between measurements of the different solutions the conductivity cell was cleaned thoroughly with Milli-Q water and rinsed with a small amount of the sample of the new solution from which conductivity data was to be measured.
After completing the first 30 measurements, homogenate was removed, and a surface of OW sensor (or the sample tray of the PA cell) was cleaned.
Two weeks later, his bone marrow, which had been full of leukemia cells, was clean, a biopsy showed.
For patch-clamp recording LC neurons were visualized by Nomarski optics using infrared light and individual cell somata were cleaned by gentle flow of aCSF from a pipette.
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