Images of stained cells were captured using a confocal microscope SP5 (Leica, USA).
The fluorescence images of cells were captured by the laser scanning confocal microscope (Leica TCS SP8 CARS, Wetzlar, Germany).
The fluorescence images of cells were captured using a commercial confocal laser scanning microscope (FV1200, Olympus, Tokyo, Japan).
The serial images of scratched monolayer cells were captured at 0 and 24 h by an inverted microscope.
Images of the lower surfaces (with migrant cells) were captured by a photomicroscope (5 fields per chamber) (BX51 Olympus, Japan), and the cells were counted blindly.
The lower surfaces (with migrant cells) were captured using a photomicroscope (5 fields per chamber) (BX51 Olympus, Japan), and the cells were counted blindly.
Finally, the scaffolds were washed twice gently with distilled water and digital images of stained cells were captured by a microscope (Nikon E 4500, Japan) fitted with camera.
The lower surfaces (with migrant cells) were captured by photomicroscopy (BX51 Olympus, Japan), and the cells were blindly counted (five fields per chamber).
A sandwich immunoassay format was utilized in which pathogenic cells were captured by antibodies immobilized onto activated carbon particles, and labeled with horseradish peroxidase (HRP) conjugated antibodies.
Images of individual cells were captured at 0% strain as well as sequentially at 2%, 4% and 6% grip-to-grip tendon strain.
It was shown that higher numbers of cells were captured across all EpCAM-positive cell lines in optimal areas versus non-optimal areas.
