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The cells were adapted for one month to attain steady-state selenium status.
First, CHO cells were adapted to grow in a commercial serum-free medium.
By knockout of rpoS in Pseudomonas aeruginosa PAO1, the biofilm formation on the electrode was enhanced and the bacterial cells were adapted to a new electron shuttle, which promoted the extracellular electron transfer and improved the performance of BES.
After adapting the undifferentiated cells to feeder-free culture on Matrigel (BD Biosciences; 08774552) in mTeSR1 (StemCell Technologies Inc.; 05850), undifferentiated cells were adapted and maintained in mTeSR1 in 500-ml spinner flasks (Corning; 89,089 814) on a stir plate (Chemglass) rotating at 70 r.p.m. in a humidified 37 °C incubator set at 5% CO2.
Culture conditions for porcine granulosa cells were adapted that maximized the selective induction of LH/CG receptors by cAMP-inducing stimuli with an elimination of background LH/CG receptor appearance.
S2R+ cells were adapted to growth in delipidated medium containing 10% lipid-extracted FBS in SDM.
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A few cells are adapted to accommodate hoists, hospital beds, and specialist mattresses.
Sheep pox virus initially adapted to replicate in primary lamb kidney cells was adapted to Vero cells by serial passages in monolayer cultures.
The upper form (designated EZH2p for primary) that is dominant in WT cells gives way to a lower form as cells are adapted to growth in culture, and this is increasingly up-regulated with passage (Fig. 5a, c).
A primary culture of choroidal epithelial cells was adapted to grow on a permeable membrane sandwiched between two culture chambers to mimic blood CSF barrier.
Different tissues and cells are adapted for specific and often diverse function, so it is important to express the genes in specific cell-types and to pyramid a range of traits.
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